Composite

Part:BBa_K180013

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)

Rock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]

iGEM2009 Team:Southampton rock-paper-scissors 'rock' secondary plasmid.

LacI is generated under rhl pR regulation.


Usage and Biology

To function as the 'rock' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180010.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1226
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
Parameters
controlrhl
directionforward
n/aRock-paper-scissors - Rock secondary plasmid [rhl pR, LacI generator]
proteinlacI